Detection Kit For Monkeypox Virus Dna (mutiplex Fluorescence Pcr)

Monkeypox is a disease caused by the monkeypox virus. It is a viral zoonotic disease, meaning that it can spread from animals to humans. It can also spread between people.

Monkeypox is commonly found in central and west Africa where there are tropical rainforests and where animals that may carry the virus typically live. People with monkeypox are occasionally identified in other countries outside of central and west Africa, following travel from regions where monkeypox is endemic.

Symptoms of monkeypox typically include a fever, intense headache, muscle aches, back pain, low energy, swollen lymph nodes and a skin rash or lesions.

The case fatality rate is 1%-10%, and effective protection can be obtained by vaccination.
Since the beginning of May 2022, multiple cases of monkeypox have been detected in several non-endemic countries, and human-to-human transmission has occurred. The virus has a great hidden danger to human health and endangers human life and health. Therefore, the rapid detection of monkeypox virus plays an important role in the prevention and quarantine of monkeypox virus.

Detection method:Multiplex Fluorescence Polymerase Chain Reaction

Applicable Disease:Monkeypox Virus

Instruments Validated:Stratagene Mx3000P™, ABI 7500,SLAN-96P and other fluorescence quantitative PCR analyzers

Sample Type:1. Skin lesions- the roof or fluid from vesicles and pustules, and dry crusts; 2. Whole blood or serum; 3. Oropharyngeal swab or Nasopharyngeal swab

Detection of specific gene sequences in monkeypox virus DNA.

High Specificity ： No cross reactivity of the assay was observed with human genome, Variola virus，Vaccinia virus etc.

Fast Results：One-step procedure, results in 70 mins

Limit of Detection（LoD）：200 copies/ML